Dr. John Nolan received B.S. degrees in biology and chemistry at the University of Illinois, Urbana-Champaign, and a Ph.D. in biochemistry from The Pennsylvania State University. After a postdoctoral position at the National Flow Cytometry Resource (NFCR), Los Alamos National Laboratory (LANL), he was promoted to the technical staff at LANL. There his lab pioneered quantitative and multiplexed flow cytometry approaches for analysis of molecular assemblies involved in catalysis, signaling, and pathogenesis, and for high throughput genomic analysis. He served as a co-investigator of the NFCR, 1996-2001, and principal investigator and director, 2001-2004. In 2004, he moved to the La Jolla Bioengineering Institute, where his lab develops instrumentation and reagents for quantitative and highly multiplexed cell and molecular analysis. His lab recently developed the first Raman flow cytometers, as well as technologies for the rapid and high resolution analysis of individual nanoparticles. Dr. Nolan serves on the editorial boards of Cytometry and Current Protocols in Cytometry, among others, and is an elected Fellow of the American Institute of Medical and Biological Engineering.